摘要 :
This study described the breeding and selection of two cultivars of Lentinula edodes, K05 and K48. Strain K05 was selected from colchicine-treated dikaryon strain 135, and strain K48 was selected from a di-mon crossing between a c...
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This study described the breeding and selection of two cultivars of Lentinula edodes, K05 and K48. Strain K05 was selected from colchicine-treated dikaryon strain 135, and strain K48 was selected from a di-mon crossing between a colchicine-treated mutant (388B1) from the original monokaryotic strain K303 and a dikaryon strain JL-2. The identification of antagonistic, esterase isozyme, molecular markers etc. revealed that strains K05 and K48 were different from the initial strain 135 and the parent strains K303 and JL-2. The production increase of new strains was notable. Compared with strain 135, the average production of new strain K05 increased by 28. 5%, and compared with the dominant cultivated strain 939, strain K48 increased by 10.7%, and also showed good quality. New strains K05 and K48 are expected to replace the conventional cultivated strains in the future. The results not only meet the requirement to the new strains for mushroom farmers but also offer a new pathway for genetic breeding in Lentinula edodes.
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摘要 :
This study described the breeding and selection of two cultivars of Lentinula edodes, K05 and K48. Strain K05 was selected from colchicine-treated dikaryon strain 135, and strain K48 was selected from a di-mon crossing between a c...
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This study described the breeding and selection of two cultivars of Lentinula edodes, K05 and K48. Strain K05 was selected from colchicine-treated dikaryon strain 135, and strain K48 was selected from a di-mon crossing between a colchicine-treated mutant (388B1) from the original monokaryotic strain K303 and a dikaryon strain JL-2. The identification of antagonistic, esterase isozyme, molecular markers etc. revealed that strains K05 and K48 were different from the initial strain 135 and the parent strains K303 and JL-2. The production increase of new strains was notable. Compared with strain 135, the average production of new strain K05 increased by 28. 5%, and compared with the dominant cultivated strain 939, strain K48 increased by 10.7%, and also showed good quality. New strains K05 and K48 are expected to replace the conventional cultivated strains in the future. The results not only meet the requirement to the new strains for mushroom farmers but also offer a new pathway for genetic breeding in Lentinula edodes.
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摘要 :
We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, c...
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We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, carboxymethyl cellucose(CMC) enzyme relative activities of 10 sporulated monocaryotic strains of Auricularia auricula. The results showed that polymorphism in the mycelial growth vigor and growth rate existed among the sporulated monocaryotic A. auricula strains tested. There was no serious deviation in the 11 ratios between mating type A and mating type a. Ten marker bands of esterase isozyme with different movement rates and 70 bands were screened in 14 tested strains. Compared with parental bicaryotic strain of Au-8,5 new bands emerged. The CMC enzyme relative activities of 10 sporulated monocaryotic strains of A. auricula were not regular. It was therefore concluded that there was no significant relationship between the mycelial growth vigor and rate with the mating type, and that significant variation was produced among the sporulated monocaryotic strains of A. auricula.
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摘要 :
We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, c...
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We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, carboxymethyl cellucose(CMC) enzyme relative activities of 10 sporulated monocaryotic strains of Auricularia auricula. The results showed that polymorphism in the mycelial growth vigor and growth rate existed among the sporulated monocaryotic A. auricula strains tested. There was no serious deviation in the 11 ratios between mating type A and mating type a. Ten marker bands of esterase isozyme with different movement rates and 70 bands were screened in 14 tested strains. Compared with parental bicaryotic strain of Au-8,5 new bands emerged. The CMC enzyme relative activities of 10 sporulated monocaryotic strains of A. auricula were not regular. It was therefore concluded that there was no significant relationship between the mycelial growth vigor and rate with the mating type, and that significant variation was produced among the sporulated monocaryotic strains of A. auricula.
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28S rDNA 5′ half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A si...
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28S rDNA 5′ half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A single uniform 1.46 kb product resulted from PCR amplification of 28S rDNA 5′ half of four genera. Amplification of ITS resulted in a single 680 bp product for Pleurotus, 700 bp for H. serotina, 720 bp for L. edodes and A. bisporus. The length of IGS1 was varied: a 1.1 kb product for P. djamor and P. salmoneostramineus, a common product 1.0 kb for the other Pleurotus isolates, 700 bp for H. serotina, 1.3 kb for L. edodes and A. bisporus. Dendrogram based on PCR-RFLP of 28S rDNA 5′ half, ITS and IGS1 suggested that H. serotina, L. edodes and A. bisporus could distinguish from Pleurotus, the isolates of Pleurotus were divided into five groups; P. tube-rregium, P. djamor and P. salmoneostramineus, P. abalonus and P. cystiodisus, P. citri-nopileatus, the other isolates clustered together. P. abalonus and P. cystidiosus were the same species, as well as P. djamor and P. salmoneostramineus, P. pulmonarius and P. sajor-caju. rDNA PCR-RFLP was more suitable for taxonomy and phylogenetic relationships of Pleurotus.
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摘要 :
28S rDNA 5′ half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A si...
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28S rDNA 5′ half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A single uniform 1.46 kb product resulted from PCR amplification of 28S rDNA 5′ half of four genera. Amplification of ITS resulted in a single 680 bp product for Pleurotus, 700 bp for H. serotina, 720 bp for L. edodes and A. bisporus. The length of IGS1 was varied: a 1.1 kb product for P. djamor and P. salmoneostramineus, a common product 1.0 kb for the other Pleurotus isolates, 700 bp for H. serotina, 1.3 kb for L. edodes and A. bisporus. Dendrogram based on PCR-RFLP of 28S rDNA 5′ half, ITS and IGS1 suggested that H. serotina, L. edodes and A. bisporus could distinguish from Pleurotus, the isolates of Pleurotus were divided into five groups; P. tube-rregium, P. djamor and P. salmoneostramineus, P. abalonus and P. cystiodisus, P. citri-nopileatus, the other isolates clustered together. P. abalonus and P. cystidiosus were the same species, as well as P. djamor and P. salmoneostramineus, P. pulmonarius and P. sajor-caju. rDNA PCR-RFLP was more suitable for taxonomy and phylogenetic relationships of Pleurotus.
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Gomphus purpuraceus is an important rare mycorrhizal edible fungus. It is very difficult to identify the isolates by fruiting. The tissue isolation from different parts of different developmental phases of fruiting body and mycorr...
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Gomphus purpuraceus is an important rare mycorrhizal edible fungus. It is very difficult to identify the isolates by fruiting. The tissue isolation from different parts of different developmental phases of fruiting body and mycorrhiza was made with 5 media. The results showed that mycelia from fruiting body grew very slowly and thickly, mycelia from mycorrhiza and rhizomorph grew quickly and thinly. Out of 5 media, medium D was optimal medium for tissue isolation. The isolates from different resources were appraised through DNA fingerprinting in comparison with that of dry basidiocarps of G. purpuraceus, and RAPD patterns were sharp and stable by 8 random primers. The results suggested that isolates from fruiting body had the same DNA fingerprinting patterns as that of the basidiocarps tissues (such as pileus and stipe), whose similarity coeffecient was 0.884. However, the RAPD patterns of the isolates from mycorrhiza and rhizomorph were different from the others (including the basidiocarps tissues). These results further revealed that some isolates from fruiting body were real isolates of G. purpuraceus and the isolates from mycorrhiza and rhizomorph were not real isolates of the G. purpuraceus.
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摘要 :
Gomphus purpuraceus is an important rare mycorrhizal edible fungus. It is very difficult to identify the isolates by fruiting. The tissue isolation from different parts of different developmental phases of fruiting body and mycorr...
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Gomphus purpuraceus is an important rare mycorrhizal edible fungus. It is very difficult to identify the isolates by fruiting. The tissue isolation from different parts of different developmental phases of fruiting body and mycorrhiza was made with 5 media. The results showed that mycelia from fruiting body grew very slowly and thickly, mycelia from mycorrhiza and rhizomorph grew quickly and thinly. Out of 5 media, medium D was optimal medium for tissue isolation. The isolates from different resources were appraised through DNA fingerprinting in comparison with that of dry basidiocarps of G. purpuraceus, and RAPD patterns were sharp and stable by 8 random primers. The results suggested that isolates from fruiting body had the same DNA fingerprinting patterns as that of the basidiocarps tissues (such as pileus and stipe), whose similarity coeffecient was 0.884. However, the RAPD patterns of the isolates from mycorrhiza and rhizomorph were different from the others (including the basidiocarps tissues). These results further revealed that some isolates from fruiting body were real isolates of G. purpuraceus and the isolates from mycorrhiza and rhizomorph were not real isolates of the G. purpuraceus.
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Saccharomyces cerevisae is one of the important experimental organisms for the eukaryotic genetics and molecular bology. Pulsed field gel electrophoresis (PFGE) has been applied in its karytytping and chromosomal DNA length polymo...
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Saccharomyces cerevisae is one of the important experimental organisms for the eukaryotic genetics and molecular bology. Pulsed field gel electrophoresis (PFGE) has been applied in its karytytping and chromosomal DNA length polymorphism (CLPs) analysis. The electrophoretic karyotype of S. cerevisae strain x 8 from the Institute of Genetics Of Academia SInica was studied on CHEF mapper equirpment.
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